PhD Position in Plants and Crops
Ghent University Department of Plants and Crops
Belgium

PhD Student in Plants and Crops

Last application date:Jul 15, 2020 14:29
Department:LA21 - Department of Plants and Crops
Employment category:Doctoral fellow
Contract:Limited duration
Degree:master of science in biology, biochemistry and biotechnology, bio-engineer or similar
Occupancy rate:100%
Vacancy Type:Research staff

Job description

“Mi(gh)ty CRISPr: developing CRISPR/Cas9 as a functional genetic tool for spider mite research”.

Background:

The two-spotted spider mite T. urticae (Arthropoda: Chelicerata) is a serious pest in agriculture. It feeds on an exceptional broad host range (over 1,100 different plant species) and is notorious for developing pesticide resistance. The molecular mechanisms underlying spider mite adaptation to xenobiotic compounds (pesticides and plant defense molecules) has been untangled to some extent. However, reliable tools for genome editing that can provide proof of principle for the involvement of genes and mutations in spider mite adaptation are currently non-existent. CRISPR/Cas9 has revolutionized functional genetic work in many organisms but, although straightforward for many model and non-model arthropods, has never been efficient in chelicerates, including spiders, scorpions and spider mites. Very recently, we have shown that the Cas9-sgRNA complex could be delivered into T. urticae oocytes after injection of T. urticae females, circumventing traditional embryo injections, but the observed CRISPR-Cas9 efficiency was very low. In this project, we want to significantly improve this efficiency and design a Cas9 delivery system, based on receptor-mediated ovary transduction (ReMOT, see https://www.nature.com/articles/s41467-018-05425-9), tailored to spider mites. The final aim is to develop this unique tool for genome editing and use it to provide proof of principle for the involvement of key factors in adaptation in this species.

Profile of the candidate

As a candidate, you have/are:

  • a MSc degree in Biology, Bioscience Engineering, Biochemistry and Biotechnology, Bio-informatics or equivalent (or will have this degree at the beginning of the project); related degrees may be considered but only when sufficient background in molecular biology and bioinformatics can be clearly demonstrated
  • well acquainted with molecular biology and/or bioinformatics
  • a drive to answer biological questions
  • an analytical, critical and independent attitude
  • excellent skills for time management and the ability to meet objectives within set deadlines.
  • demonstrated ability to work efficiently and with minimum supervision.
  • proficient in English, both written and oral

Ideally, you have a strong interest or experience in/with genetic transformation of arthropods, protein expression or analysis of sequencing data. We provide an open and stimulating working environment, in which teamwork, initiative, a critical mindset and originality are highly appreciated. We offer a position for 1 year, which will normally be extended to 4 years in case of a positive evaluation.

How to apply

Interested candidates should submit an application no later than 15th of July 2020 to Leen Dierick (Leen.Dierick@ugent.be). The application should contain 1/ a motivation letter to introduce yourself and to highlight the key points you should be considered for the position; 2/ a curriculum vitae including educational qualifications, employment history if applicable, research fields and current




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