Research Fellow in Mechanistic Cell Biology University of Leeds - Faculty of Biological Sciences United Kingdom

Are you an ambitious researcher looking for your next challenge? Do you have an established background in cell biology and biochemistry? Do you want to further your career in one of the UKs leading research intensive Universities? Do you have an interest in understanding the molecular basis of autophagy regulation?

Based in state-of-the-art laboratory space, and under the supervision of Dr. Natalia Riobo-Del Galdo you will work on a project which aims to understand “the molecular mechanism of regulation of autophagy by the Hedgehog pathway receptor and tumor suppressor PTCH1”, funded by a BBSRC project grant.

The project aims to uncover how PTCH1’s biochemical activity as a cholesterol transporter can impair the last stage of autophagy. This project will rely heavily in cell culture, using transfection, shRNA-mediated silencing, CRISPR/Cas9 genome editing and pharmacological treatments. This will require the generation of mutant variants of PTCH1 and other cholesterol handling proteins, as well as performing in vitro biochemical assays, expression and purification of proteins from E. coli, pulldown assays, western blotting (WB), adeno and retrovirus generation. Live and fixed cell confocal imaging techniques will be performed to detect cholesterol and pH changes with fluorescent reporters subcellularly in autolysosomes and lysosomes and colocalization of candidate proteins in autophagosomes. 

You should have or be close to completing a PhD in cell and/or molecular biology or biochemistry of transporter proteins. You will have extensive practical experience in imaging, cell culture techniques, and biochemical assays. Previous work in autophagy and/or lysosomal storage disorders is required. Knowledge of protein purification, cholesterol handling or Hedgehog signalling is desirable, but not essential.

The University of Leeds and the Faculty of Biological Sciences are committed to providing equal opportunities for all and offer a range of family friendly policies. The University is a charter member of Athena SWAN (the national body that promotes gender equality in higher education), and the Faculty of Biological Sciences was reawarded a Bronze award in 2017.  We are proud to be an inclusive Faculty that values all staff, and are happy to consider job share applications and requests for flexible working arrangements from our employees. Our Athena SWAN webpage provides more information.

To explore the post further or for any queries you may have, please contact:

Dr Natalia Riobo-Del Galdo, Group Leader – The Hedgehog Lab     

Tel: +44 (0)113 343 9184, email: n.a.riobo-delgaldo@leeds.ac.uk 

 

Location: Leeds - Main Campus

Faculty/Service: Faculty of Biological Sciences

School/Institute: School of Molecular & Cellular Biology

Category: Research

Grade: Grade 7

Salary: £33,199 to £39,609 p.a.

 

Due to funding limitations it is unlikely that an appointment will be made above £35,211 p.a.

Working Time: 100%

Post Type: Full Time

Contract Type: Fixed Term (3 years - external funding)

Release Date: Wednesday 10 July 2019

Closing Date: Friday 09 August 2019

Reference: FBSMB1161

 


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