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Employment1.0 FTE
Gross monthly salary€ 2,770 - € 3,539
Required backgroundResearch University Degree
Organizational unitFaculty of Science
Application deadline10 September 2023
Are you a team-oriented PhD candidate with an interest in molecular biology? And would you like to learn and work in a multidisciplinary team in an innovative laboratory environment as well as be part of a Marie Curie Doctoral Network? If so, come and Join the lively and world-class RIMLS-Science Institute and the ChromRare network to conduct research towards the unveiling of the molecular basis of chromatinopathies.
As a member of the Department of Molecular Biology, you will enter our four-year PhD programme at Radboud University and participate in the ChromRare consortium. This consortium will focus on 'Chromatinopathies' (CPs), which are syndromes caused by mutations in chromatin-associated proteins. In the context of this network, we are looking for a PhD candidate who is eager to join our team to apply an 'off-the-shelf' proximity biotinylation enzyme called ProteinA-TurboID that was recently developed in our lab (Santos-Barriopedro et al., Nature Commun. 2021, Nature Protocols 2022). This recombinant proximity biotinylation enzyme can be targeted to baits of interest in primary cells using bait-specific antibodies, which are available for all the mutant enzymes studied in this network. This approach thus represents an ideal system to probe the proximal proteome for various mutated chromatin proteins in CPs, making use of primary patient cells or patient derived iPSC models without the need for genetic manipulation of the target cells. Identified candidate proximal proteins will be functionally verified using ChIP-seq and a technology recently developed in our lab called BANC-seq (Neikes et al., Nature Biotechnolgoy 2023). Furthermore, functional follow-up studies will be pursued such as CRISPR-based functional studies, including degron lines and RNA-seq, which we have ample experience with (Grand et al., Nature 2021). This work may provide new leads towards novel therapies for CPs. Profile
We areThe Department of Molecular Biology is part of the Radboud Institute for Molecular Life Sciences(RIMLS-Sience), which is one of the six research Institutes of the Faculty of Science at Radboud University. The overarching aim of RIMLS-Science is to decipher molecular mechanisms of disease. Research areas of RIMLS-Science include epigenetics, stem-cell and developmental biology, and computational biology. The central research theme is regulation of gene expression during development and in health and disease. Important tools used at the institute include next generation sequencing, mass spectrometry, single-cell multi-omics analysis and computational analysis and method development. To perform state-of-the-art research, RIMLS-Science operates its own wet lab and computational facilities. This fundamental and applied research in medical biology and molecular life sciences has clear links to biochemical research performed at the Institute for Molecules and Materials and other institutes of the Faculty of Science as well as to more clinically oriented research programmes at departments within the Radboud university medical center. RIMLS-Science actively contributes to teaching activities in the Biology study programme at Radboud University. RIMLS-Science currently consists of about 50 researchers (including 30 PhD candidates and postdocs) and provides a vivid and international working environment. The institute actively participates in many European and worldwide collaborations.
We are keen to meet critical thinkers who want to look closer at what really matters. People who, from their expertise, wish to contribute to a healthy, free world with equal opportunities for all. This ambition unites more than 24,000 students and 5,600 employees at Radboud University and requires even more talent, collaboration and lifelong learning. You have a part to play! |
