Researcher in Biology (Tufts University)

Overview The Department of Immunology maintains research programs focusing on translational and basic research in areas relevant to human disease. The department’s goal is to become a world-class center for the study of inflammation and the targeted development of novel therapeutics for both treatment and prevention of immune-mediated diseases. What You'll Do We are seeking a Research Technician to support studies in T-cell immunology and autoimmunity. The lab focuses on: Generating and stabilizing regulatory T (Treg) cells from effector CD4⁺ T cells. Dissecting epigenetic and metabolic pathways that govern Treg lineage stability and fitness (e.g., OXPHOS/heme biosynthesis). Testing antigen-specific Treg therapies in mouse models of multiple sclerosis (EAE). The position involves: Primary T-cell isolation and culture Differentiation assays (Treg/Th17) Viral transduction and CRISPR/Cas9 perturbations Multi-color flow cytometry Mouse colony support and EAE studies (induction, clinical scoring, tissue processing) Standard molecular biology (qPCR, cloning, Western blotting) Additional responsibilities: Keep accurate experimental records Perform basic data analyses (FlowJo, GraphPad Prism; optional R/Python) Maintain lab operations and regulatory compliance Participate in the weekly lab Journal Club Opportunities: Hands-on training, authorship commensurate with contribution, and growth toward increasing independence. Commitment: A two-year appointment is preferred. Key Responsibilities Conduct bench research experiments following protocols to achieve results Participate in animal experiments Record results and maintain accurate experimental records Perform limited data interpretation and assist with basic statistics and analysis Maintain laboratory equipment and manage inventory of supplies Prepare stock solutions, reagents, and other media What We're Looking For Basic Requirements: Bachelor’s degree in Biology, Immunology, Molecular Biology, Bioengineering, or related field Up to 1 year of relevant lab experience (including coursework); strong sterile technique and attention to detail Comfort working with mice and willingness to perform animal procedures after training Proficiency with basic molecular/biochemical techniques (pipetting, PCR, gel electrophoresis) and basic data analysis (Excel/Prism; maintaining an electronic lab notebook) Strong organizational and communication skills, reliability, initiative, ability to follow SOPs precisely Ability to accommodate occasional time-sensitive experiments outside standard hours Preferred Qualifications: Experience with primary T-cell isolation and culture; Treg/Th17 polarization assays Mouse handling and procedures (injections, EAE induction, clinical scoring, perfusion, tissue harvest) Flow cytometry (panel design, compensation, analysis in FlowJo) and/or FACS experience CRISPR/Cas9 editing; lentiviral/retroviral vector cloning and transduction qPCR, Western blotting, ELISA, RNA isolation; basic cloning Experience with data analysis tools (GraphPad Prism; optional R/Python) and figure preparation Prior exposure to IACUC/IBC protocols and BSL-2 practices Interest in a two-year commitment and growth toward semi-independent project ownership Pay Range Minimum: $20.50/hour Midpoint: $24.40/hour Maximum: $28.30/hour Salary is based on related experience, expertise, and internal equity; new hires can generally expect pay between the minimum and midpoint. If you apply for this position please say you saw it on Bioloxy

Overview

The Department of Immunology maintains research programs focusing on translational and basic research in areas relevant to human disease. The department’s goal is to become a world-class center for the study of inflammation and the targeted development of novel therapeutics for both treatment and prevention of immune-mediated diseases.

What You'll Do

We are seeking a Research Technician to support studies in T-cell immunology and autoimmunity. The lab focuses on:

Generating and stabilizing regulatory T (Treg) cells from effector CD4⁺ T cells.
Dissecting epigenetic and metabolic pathways that govern Treg lineage stability and fitness (e.g., OXPHOS/heme biosynthesis).
Testing antigen-specific Treg therapies in mouse models of multiple sclerosis (EAE).

The position involves:

Primary T-cell isolation and culture
Differentiation assays (Treg/Th17)
Viral transduction and CRISPR/Cas9 perturbations
Multi-color flow cytometry
Mouse colony support and EAE studies (induction, clinical scoring, tissue processing)
Standard molecular biology (qPCR, cloning, Western blotting)

Additional responsibilities:

Keep accurate experimental records
Perform basic data analyses (FlowJo, GraphPad Prism; optional R/Python)
Maintain lab operations and regulatory compliance
Participate in the weekly lab Journal Club

Opportunities: Hands-on training, authorship commensurate with contribution, and growth toward increasing independence.
Commitment: A two-year appointment is preferred.

Key Responsibilities

Conduct bench research experiments following protocols to achieve results
Participate in animal experiments
Record results and maintain accurate experimental records
Perform limited data interpretation and assist with basic statistics and analysis
Maintain laboratory equipment and manage inventory of supplies
Prepare stock solutions, reagents, and other media

What We're Looking For

Basic Requirements:

Bachelor’s degree in Biology, Immunology, Molecular Biology, Bioengineering, or related field
Up to 1 year of relevant lab experience (including coursework); strong sterile technique and attention to detail
Comfort working with mice and willingness to perform animal procedures after training
Proficiency with basic molecular/biochemical techniques (pipetting, PCR, gel electrophoresis) and basic data analysis (Excel/Prism; maintaining an electronic lab notebook)
Strong organizational and communication skills, reliability, initiative, ability to follow SOPs precisely
Ability to accommodate occasional time-sensitive experiments outside standard hours

Preferred Qualifications:

Experience with primary T-cell isolation and culture; Treg/Th17 polarization assays
Mouse handling and procedures (injections, EAE induction, clinical scoring, perfusion, tissue harvest)
Flow cytometry (panel design, compensation, analysis in FlowJo) and/or FACS experience
CRISPR/Cas9 editing; lentiviral/retroviral vector cloning and transduction
qPCR, Western blotting, ELISA, RNA isolation; basic cloning
Experience with data analysis tools (GraphPad Prism; optional R/Python) and figure preparation
Prior exposure to IACUC/IBC protocols and BSL-2 practices
Interest in a two-year commitment and growth toward semi-independent project ownership